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Dr. Dooley uses her training in combinatorial libraries and fluorescent imaging to research new methods for pain relief, addiction and food regulation, and investigates cells' response to stresses which may lead to a better understanding of pain management, chronic pain, addiction, Parkinson's and Alzheimer's.
Dr. Colette Dooley completed her undergraduate studies with honors in 1987 and earned her Masters of Science degree in Zoology ("Identification of binding sites for Atrial Natriuretic Peptide on Gills of Salmonids"), both at the University College Dublin, Ireland. She joined the Torrey Pines Institute in 1990 as a Research Assistant and her research included developing high throughput radioreceptor screening assays and modifying existing opioid receptor assays for screening with combinatorial libraries. In 1994, she was named a Senior Scientist at TPIMS and supervised five research technicians on projects involving the use of mixture based combinatorial libraries in radioreceptor assays for opioid ligands, melanocortin ligands, melanin concentrating hormone, substance P and orphanin FQ and is now the lead author of a study on compounds that are in clinical trials. These research projects were funded by either NIH or commercial enterprises. In 2000, she pursued her Ph.D. in the Department of Chemistry and Biochemistry at the University of California in San Diego. She trained with Roger Tsien of UCSD who is renowned for discovering calcium sensitive fluorescent dyes and optimizing Green Fluorescent Protein in 1994. She achieved her Ph.D. in Chemistry, with her thesis entitled, "Green Fluorescent Protein Based Indicators of Dynamic Redox Changes and Reactive Oxygen Species" in 2006. Dr. Dooley rejoined TPIMS in their new endeavor in Florida as an Assistant Member.
- 1987 - Bachelor of Science (Honors), Zoology, Univ. College Dublin, Ireland
- 1992 - Master of Science, Zoology, Univ. College Dublin, Ireland
- 2006 - Ph. D, Chemistry, University of California, San Diego
- 2007 - Post Doctoral, Chemistry, University of California, San Diego
- 1983-1987 Undergraduate Studies: Department of Zoology, University College Dublin, Ireland
- 1987-1990 Graduate Studies Department of Zoology University College Dublin, Ireland
- 1990-1994 Research Assistant, Torrey Pines Institute for Molecular Studies, San Diego, CA
- 1994-2000 Senior Scientist, Torrey Pines Institute for Molecular Studies, San Diego, CA
- 2000-2006 Graduate Studies, Department of Chemistry and Biochemistry UCSD
- 2006-2007 Postdoctoral Research Department of Pharmacology UCSD
- 2007- Present Assistant Member, Torrey Pines Institute for Molecular Studies
Honors & Awards
Selected Publications (10 of 55)
- Reilley KJ, Giulianotti M, Dooley CT, Nefzi A, McLaughlin JP, Houghten RA. AAPS J. Identification of Two Novel, Potent, Low-Liability Antinociceptive Compounds from the Direct In Vivo Screening of a Large Mixture-Based Combinatorial Library. (2010) 12 318-329 PMID: 20422341
- Nagasaki H, Chung S, Dooley CT, Wang Z, Li C, Saito Y, Clark SD, Houghten RA, Civelli O The pharmacological properties of a novel MCH(1) receptor antagonist isolated from combinatorial libraries. Eur J. Pharmacology (2009) 14 192-202 PMID: 19041642
- Houghten, Richard A.; Pinilla, Clemencia; Giulianotti, Marc A.; Appel, Jon R.; Dooley, Colette T.; Nefzi, Adel; Ostresh, John M.; Yu, Yongping; Maggiora, Gerald M.; Medina-Franco, Jose L.; Brunner, Daniela; Schneider, Jeff. Strategies for the Use of Mixture-Based Synthetic Combinatorial Libraries: Scaffold Ranking, Direct Testing In Vivo, and Enhanced Deconvolution by Computational Methods. Journal of Combinatorial Chemistry (2008), 10(1), 3-19. PMID: 18067268
- In vitro and direct in vivo testing of mixture-based combinatorial libraries for the identification of highly active and specific opiate ligands. Houghten RA, Dooley CT, Appel JR. AAPS J. 2006 May 26;8(2):E371-82. Review. PMID: 16796388
- Dooley,C.T., Dore,T.M., Hanson,G.T., Jackson,W.C., Remington,S.J., Tsien, R.Y. (2004) Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators. J. Biol. Chem 279 22284-22293. PMID: 14985369
- Dooley, C. T., Ny, P., Bidlack, J. M., and Houghten, R. A. (1998). Selective ligands for the μ, δ and κ opioid receptors identified from a single tetrapeptide positional scanning combinatorial library. Journal of Biological Chemistry 273, 18848‑18856. PMID: 9668060
- Binding and in vitro activities of peptides with high affinity for the nociceptin/orphanin FQ receptor, ORL1. Dooley CT, Spaeth CG, Berzetei-Gurske IP, Craymer K, Adapa ID, Brandt SR, Houghten RA, Toll L. J Pharmacol Exp Ther. 1997 Nov;283(2):735-41. PMID: 9353393
- Dooley, C. T., Chung, N. N., Wilkes, B. C., Schiller, P. W., Bidlack, J. M., Pasternak, G. W.,Houghten, R. A.. An all D‑amino acid opioid peptide with central analgesic activity from a combinatorial library. Science 266, (1994) 2019‑2022. PMID: 7801131
- Dooley, C. T., Chung, N. N., Schiller, P. W., and Houghten, R. A. (1993). Acetalins: Opioid receptor antagonists determined through the use of synthetic peptide combinatorial libraries. PNAS 90, 10811‑ 10815. PMID: 8248174
- Houghten, R. A., Pinilla, C., Blondelle, S. E., Appel, J. R., Dooley, C. T., and Cuervo, J. H. (1991). Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery. Nature 354, 84‑86. PMID: 1719428
Patents (5 of 8)
- Melanin-concentrating hormone receptor antagonists and methods of use. United States of America US 2004-555820P issued 2004 WO2005-US9527 PCT Int. Appl. Issued 2005. Inventors, Civelli, Olivier; Nagasaki, Hiroshi; Houghten, Richard A.; Dooley, Colette; Nefzi, Adel.
- Combinatorial libraries of bicyclic guanidine derivatives and compounds therein, United States of America, 6359144, Issued March19, 2002. Inventors: J.M. Ostresh, J.P. Meyer, C.T. Dooley, S.E. Blondelle, C.C. Schoner, R.A. Houghten.
- Melanocortin receptor ligands and methods of using same, United States of America, 6350430, issued February 26, 2002. Inventors: C.T. Dooley, B.E. Girten and R.A. Houghten.
- Novel mu opioid receptor ligands: Agonists and antagonists. U.S. Patent No. 5,641,861. Issued June 24,1997. Inventors: C.T. Dooley and R.A. Houghten.
- Novel kappa receptor selective opioid peptides. U.S. Patent No. 5,610,271. Issued March 11, 1997. Inventors: C.T. Dooley and R.A. Houghten.