Torrey Pines Institute for Molecular Studies science image
Torrey Pines Institute for
Molecular Studies
5775 N. Old Dixie Highway
Fort Pierce, FL 34946-7302
USA
Scientists
Colette Dooley
Assistant Member
Pain Management

772.462.0889 - phone
772.462.0886 - fax

Dr. Colette Dooley completed her undergraduate studies with honors in 1987 and earned her Masters of Science degree in Zoology ("Identification of binding sites for Atrial Natriuretic Peptide on Gills of Salmonids"), both at the University College Dublin, Ireland. She joined the Torrey Pines Institute in 1990 as a Research Assistant and her research included developing high throughput radioreceptor screening assays and modifying existing opioid receptor assays for screening with combinatorial libraries. In 1994, she was named a Senior Scientist at TPIMS and supervised five research technicians on projects involving the use of mixture based combinatorial libraries in radioreceptor assays for opioid ligands, melanocortin ligands, melanin concentrating hormone, substance P and orphanin FQ and is now the lead author of a study on compounds that are in clinical trials. These research projects were funded by either NIH or commercial enterprises. In 2000, she pursued her Ph.D. in the Department of Chemistry and Biochemistry at the University of California in San Diego. She trained with Roger Tsien of UCSD who is renowned for discovering calcium sensitive fluorescent dyes and optimizing Green Fluorescent Protein in 1994. She achieved her Ph.D. in Chemistry, with her thesis entitled, "Green Fluorescent Protein Based Indicators of Dynamic Redox Changes and Reactive Oxygen Species" in 2006. Dr. Dooley rejoined TPIMS in their new endeavor in Florida as an Assistant Member.

Research Focus

Dr. Dooley uses her training in combinatorial libraries and fluorescent imaging to research new methods for pain relief, addiction and food regulation, and investigates cells' response to stresses which may lead to a better understanding of chronic pain, addiction, Parkinson's and Alzheimer's.

Publications

  1. Houghten, R.A., Dooley, C.T., Appel, J.R. In vitro and direct in vivo testing of mixture-based combinatorial libraries for the identification of highly active and specific opiate ligands. The AAPS Journal 8:E371-382, 2006.
  2. Houghten, R.A., Dooley, C.T., Appel, J.R. Mixture-based combinatorial libraries. Handbook of Biologically Active Peptides, 1478-1494, 2006.
  3. Nefzi, A., Ostresh, J.M., Appel, J.R., Bidlack, J., Dooley, C.T., Houghten, R.A. Identification of potent and highly selective chiral tri-amine and tetra-amine u opoid receptors ligands. An example of lead optimization using mixture based libraries. Bioorg. Med. Chem. Lett. 16:4331-4338, 2006.
  4. Houghten, R.A., Dooley, C.T., Appel, J.R. De novo identification of highly active fluorescent kappa opioid ligands from a rhodamine labeled tetrapeptide positional scanning library. Bioorg. Med. Chem. Lett. 14:1947-1951, 2004.
  5. Dooley, C.T., Mabini, A., Sylvester, T., Appel, J.R., Houghten, R.A. Identification of novel ligands for vasopressin V2 receptor from positional scanning libraries. In: Peptides: The Wave of the Future. Proceedings of the 2nd International/17th American Peptide Symposium. (Houghten, R.A., Lebl, M., Eds.), Kluwer Academic Publishers, Dordrecht, pp. 921-922, 2001.
  6. Nefzi, A., Giulianotti, M.A., Ong, N.A., Ostresh, J.M., Dooley, C.T., Blondelle, S.E., Houghten, R.A. Solid phase synthesis of acyclic and heterocyclic combinatorial libraries from resin-bound triamines. In: Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries 2000 (Epton, R., Ed.), Mayflower Worldwide, Birmingham, UK, pp. 119-122, 2001.
  7. Dooley, C.T., Houghten, R.A. New opioid peptides, peptidomimetic, and heterocyclic compounds from combinatorial libraries. Biopolymers (Peptide Science) 51:379-390, 2000.
  8. Dooley, C.T., Houghten, R.A. Orphanin FQ/Nociceptin receptor binding studies. Peptides 21:949-960, 2000.
  9. Pinilla, C., Appel, J.R., Blondelle, S.E., Dooley, C.T., Eichler, J., Nefzi, A., Ostresh, J.M., Martin, R., Wilson, D.B., Houghten, R.A. Synthesis and screening of positional scanning synthetic combinatorial libraries. In: Combinatorial Chemistry: A Practical Approach (Fenniri, H., Ed.), Oxford University Press, Oxford, pp. 51-74, 2000.
  10. Dooley, C.T., Houghten, R.A. New opioid peptides, peptidomimetics, and heterocyclic compounds from combinatorial libraries. Biopolymers 51:379-390, 1999.
  11. Houghten, R.A., Dooley, C.T., Giulianotti, M.A., Hamashin, V.T., Schoner, C., Ostresh, J.M., Nefzi, A. Solid phase synthesis of heterocyclic positional scanning combinatorial libraries. In: Innovation and Perspectives in Solid Phase Synthesis and Combinatorial Libraries 1998 (Epton, R., Ed.), Mayflower Worldwide Ltd., Birmingham,UK, pp. 11-14, 1999.
  12. Houghten, R.A., Pinilla, C., Appel, J.R., Blondelle, S.E., Dooley, C.T., Eichler, J., Nefzi, A., Ostresh, J.M. Mixture-based synthetic combinatorial libraries. J. Med. Chem. 42:3743-3778, 1999.
  13. Dooley, C.T., Houghten, R.A. Synthesis and screening of positional scanning combinatorial libraries. In: Methods In Molecular Biology: Combinatorial Peptide Library Protocols (Cabilly, S., Eds.), Humana Press, Inc., New Jersey, pp. 13-24, 1998.
  14. Dooley, C.T., Ny, P., Bidlack, J.M., Houghten, R.A. Selective ligands for the μ, δ, and κ opioid receptors identified from a single tetrapeptide positional scanning combinatorial library. J. Biol. Chem. 273:18848-18856, 1998.
  15. Dooley, C.T., Spaeth, C.G., Toll, L., Berzetei-Gurske, I.P., Houghten, R.A. Potent Orphanin FQ receptor ligands identified using combinatorial libraries. In: Peptides 1996, Proceedings of the Twenty-Fourth European Peptide Symposium (Ramage, R., Epton, R., Eds.), Mayflower Scientific Ltd., Kingswinford, UK, pp. 343-344, 1998.
  16. Eichler, J., Blondelle, S.E., Dooley, C.T., Houghten, R.A. Scaffolded peptide combinatorial libraries for the design of proteinmimetics. In: Peptides 1996: Proceedings of the Twenty-Fourth European Peptide Symposium (Ramage, R., Epton, R., Eds.), Mayflower Scientific Ltd., Kingswinford, UK, pp. 361-362, 1998.
  17. Houghten, R.A., Blondelle, S.E., Dooley, C.T., Meyer, J.-P., Ostresh, J.M., Nefzi, A. Preparation of heterocyclic positional scanning combinatorial libraries from peptides. In: Peptide Science - Present and Future (Shimonishi, Y., Ed.), Kluwer Academic Publishers, The Netherlands, pp. 125-129, 1998.
  18. Nefzi, A., Dooley, C.T., Ostresh, J.M., Houghten, R.A. Combinatorial chemistry: from peptides and peptidomimetics to small organic and heterocyclic compounds. Bioorg. Med. Chem. Lett. 8:2273-2278, 1998.
  19. Dooley, C.T., Spaeth, C.G., Craymer, K., Adapa, I.D., Brandt, S.R., Houghten, R., Toll, L. Binding and in vitro activities of peptides with high affinity for the Nociceptin/Orphanin FQ receptor, ORL1. J. Pharmacol. Exp. Ther. 283:735-741, 1997.
  20. Dörner, B., Ostresh, J.M., Blondelle, S.E., Dooley, C.T., Houghten, R.A. Peptidomimetic synthetic combinatorial libraries. In: Advances in Amino Acid Mimetics and Peptidomimetics (Abell, A., Ed.), JAI Press, Greenwich, CT, pp. 109-125, 1997.
  21. Kiely, J.S., Dörner, B., Ostresh, J.M., Dooley, C.T., Houghten, R.A. Rapidly Expanding Molecular Diversity: Libraries from Libraries. In: High Throughput Screening: The Discovery of Bioactive Substances (Devlin, J., Ed.), Marcel-Dekker, New York, pp. 155-166, 1997.
  22. Dooley, C.T., Bower, A.N., Houghten, R.A. Identification of mu-selective tetrapeptides using a positional scanning combinatorial library containing L-, D- and unnatural amino acids. In: Peptides: Chemistry, Structure and Biology (Proceedings of the Fourteenth American Peptide Symposium) (Kaumaya, P. T. P. and Hodges, R. S., Eds.), ESCOM, Leiden, 1996.
  23. Dooley, C.T., Houghten, R.A. Orphanin FQ: receptor binding and analog structure activity relationships in rat brain. Life Sci. 59:PL23-PL29, 1996.
  24. Dörner, B., Blondelle, S.E., Pinilla, C., Appel, J., Dooley, C.T., Eichler, J., Ostresh, J.M., Pérez-Payá, E., Houghten, R.A. Soluble synthetic combinatorial libraries: The use of molecular diversities for drug discovery. In: Combinatorial Libraries. Synthesis, Screening and Application Potential (Cortese, R., Ed.), Walter de Gruyter & Co., New York, pp. 1-25, 1996.
  25. Griffith, M.C., Dooley, C.T., Houghten, R.A., Kiely, J.S. Solid-phase synthesis, characterization, and screening of a 43,000-compound tetrahydroisoquinoline combinatorial library. In: Molecular Diversity and Combinatorial Chemistry: Libraries and Drug Discovery (Chaiken, I. M. and Janda, K. D., Eds.), American Chemical Society, Washington, DC, pp. 50-57, 1996.
  26. Houghten, R.A., Blondelle, S.E., Dooley, C.T., Dörner, B., Eichler, J., Ostresh, J.M. Libraries from libraries: Generation and comparison of screening profiles. Mol. Div. 2:41-45, 1996.
  27. Houghten, R.A., Dooley, C.T., Ostresh, J.M. The use of soluble polyamine combinatorial libraries for the identification of potent opioid receptor active compounds. In: Peptides: Chemistry, Structure and Biology, Proceedings of the Fourteenth American Peptide Symposium (Kaumaya, P.T.P. and Hodges, R.S., Eds.), Mayflower Scientific, Ltd., England, pp. 278-280, 1996.
  28. Pinilla, C., Appel, J.R., Blondelle, S.E., Dooley, C.T., Dörner, B., Eichler, J., Ostresh, J.M., Houghten, R.A. Synthetic combinatorial libraries screened in solution. In: Peptides: Chemistry, Structure and Biology, Proceedings of the Fourteenth American Peptide Symposium (Kaumaya, P.T.P. and Hodges, R.S., Eds.), Mayflower Scientific, Ltd., Kingswinford, UK, pp. 884-886, 1996.
  29. Pinilla, C., Appel, J.R., Dooley, C.T., Blondelle, S.E., Eichler, J., Dörner, B., Ostresh, J.M., Houghten, R.A. The versatility of nonsupport-bound combinatorial libraries. In: Combinatorial Peptide and Nonpeptide Libraries (Jung, G., Ed.), Verlag Chemie, Wienheim, pp. 139-171, 1996.
  30. Blondelle, S.E., Pérez-Payá, E., Dooley, C.T., Pinilla, C., Houghten, R.A. Soluble combinatorial libraries of organic, peptidomimetic and peptide diversities. Trends Anal. Chem. 14:83-92, 1995.
  31. Dooley, C.T., Hope, S.K., Houghten, R.A. Identification of tetrameric opioid peptides from a combinatorial library composed of L-, D- and non-proteinogenic amino acids. In: Peptides 94: Proceedings of the 23rd European Peptide Symposium (Maia, H. L. S., Eds.), ESCOM, Leiden, pp. 805-806, 1995.
  32. Dooley, C.T., Houghten, R.A. A comparison of combinatorial library approaches for the study of opioid compounds. Perspectives in Drug Discovery and Design 2:287-304, 1995.
  33. Dooley, C.T., Houghten, R.A. Identification of mu-selective polyamine antagonists from a synthetic combinatorial library. Analgesia 1:400-404, 1995.
  34. Dooley, C.T., Kaplan, R.A., Chung, N.N., Schiller, P.W., Bidlack, J.M., Houghten, R.A. Six highly active mu-selective opioid peptides identified from two synthetic combinatorial libraries. Pept. Res. 8:124-137, 1995.
  35. Eichler, J., Appel, J.R., Blondelle, S.E., Dooley, C.T., Dörner, B., Ostresh, J.M., Pérez-Payá, E., Pinilla, C., Houghten, R.A. Peptide, peptidomimetic and organic synthetic combinatorial libraries. Med. Res. Rev. 15:481-496, 1995.
  36. Pinilla, C., Appel, J.R., Blondelle, S.E., Dooley, C.T., Dörner, B., Eichler, J., Ostresh, J.M., Houghten, R.A. A review of the utility of peptide combinatorial libraries. Biopolymers (Peptide Science) 37:221-240, 1995.
  37. Dooley, C.T., Chung, N.N., Wilkes, B.C., Schiller, P.W., Bidlack, J.M., Pasternak, G.W., Houghten, R.A. An all D-amino acid opioid peptide with central analgesic activity from a combinatorial library. Science 266:2019-2022, 1994.
  38. Dooley, C.T., Hope, S., Houghten, R.A. Rapid identification of novel opioid peptides from an N-acetylated synthetic combinatorial library. Regul. Pept. 54:87-88, 1994.
  39. Dooley, C.T., Houghten, R.A. New, potent N-acetylated all D-amino acid opioid peptides. In: Peptides: Chemistry, Structure and Biology. Proceedings of the 13th American Peptide Symposium (RS Hodges and JA Smith, Eds.) ESCOM, Leiden, pp. 984-985, 1994.
  40. Houghten, R.A., Appel, J.R., Blondelle, S.E., Cuervo, J.H., Dooley, C.T., Eichler, J., Pinilla, C. Optimal peptide length determination using synthetic peptide combinatorial libraries. In: Peptides: Proceedings of the 13th American Peptide Symposium (RS Hodges and JA Smith, Eds.) ESCOM, Leiden, pp. 971-974, 1994.
  41. Pinilla, C., Appel, J.R., Blondelle, S.E., Dooley, C.T., Eichler, J., Ostresh, J.M., Houghten, R.A. Versatility of positional scanning synthetic combinatorial libraries for the identification of individual compounds. Drug Dev. Res. 33:133-145, 1994.
  42. Dooley, C.T., Chung, N.N., Schiller, P.W., Houghten, R.A. Acetalins: Opioid receptor antagonists determined through the use of synthetic peptide combinatorial libraries. Proc. Natl. Acad. Sci. USA 90:10811-10815, 1993.
  43. Dooley, C.T., Houghten, R.A. The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands. Life Sci. 52:1509-1517, 1993.
  44. Dooley, C.T., Prochera, A.M., Houghten, R.A. A priori determination of peptide ligands for opiate receptors using synthetic peptide combinatorial libraries. In: Peptides 1992: Proceedings of the 22nd European Peptide Symposium (CH Schneider and AN Eberle, Eds.) ESCOM Science Publishers B.V. Leiden, pp. 649-650, 1993.
  45. Houghten, R.A., Dooley, C.T. The use of synthetic peptide combinatorial libraries for the determination of peptide ligands in radio-receptor assays: opioid peptides. Bioorg. Med. Chem. Lett. 3:405-412, 1993.
  46. Houghten, R.A., Dooley, C.T. Novel N-acetylated opioid peptides determined through the use of synthetic peptide combinatorial libraries. In: Peptide Chemistry 1992: Proceedings of the 2nd Japan Symposium on Peptide Chemistry (N Yanaihara, Ed.) ESCOM Science Publishers B.V. Leiden, pp. 11-13, 1993.
  47. Houghten, R.A., Dooley, C.T. Synthetic peptide combinatorial libraries may be used to determine peptide ligands for opioid receptors. In: Nida Rearch Monograph Series.  Vol. 134. pp.66-74 (NIH: Rockville, Md.), 1993.
  48. Houghten, R.A., Appel, J.R., Blondelle, S.E., Cuervo, J.H., Dooley, C.T., Pinilla, C. The use of synthetic peptide combinatorial libraries for the identification of bioactive peptides. Biotechniques 13:412-421, 1992.
  49. Houghten, R.A., Appel, J.R., Blondelle, S.E., Cuervo, J.H., Dooley, C.T., Pinilla, C. The use of synthetic peptide combinatorial libraries for the identification of bioactive peptides. Pept. Res. 5:351-358, 1992.
  50. Houghten, R.A., Cuervo, J.H., Pinilla, C., Appel, J.R., Dooley, C.T., Blondelle, S.E. The use of a peptide library composed of 34 012 224 hexamers for basic research and drug discovery. In: Peptides, Proceedings of the Twelfth American Peptide Symposium (JA Smith and JE Rivier, Eds.) ESCOM Science Publishers B.V. Leiden, pp. 560-561, 1992.
  51. Houghten, R.A., Pinilla, C., Blondelle, S.E., Appel, J.R., Dooley, C.T., Cuervo, J.H. Generation and use of synthetic combinatorial libraries for basic research and drug discovery. Nature 354:84-86, 1991.

Patents

  1. Combinatorial libraries of bicyclic guanidine derivatives and compounds therein. U.S. Patent No. 6,359,144. Issued March 19, 2002.  Inventors:  Ostresh, J.M., Meyer, J.-P., Dooley, C.T., Blondelle, S.E., Schoner, C.C., Houghten, R.A.
  2. Melanocortin receptor ligands and methods of using same. U.S. Patent No. 6,350,430. Issued February 26, 2002.  Inventors:  Dooley, C.T., Girten, B.E., Houghten, R.A.
  3. Selectively N-alkylated peptidomimetic combinatorial libraries and compounds therein. U.S. Patent No. 6,143,932. Issued November 7, 2000.  Inventors:  Dörner, B., Ostresh, J.M., Dooley, C.T., Houghten, R.A., Eichler, J.
  4. Selectively N-alkylated peptidomimetic combinatorial libraries and compounds therein. U.S. Patent No. 6,121,489. Issued September 19, 2000.  Inventors:  Dörner, B., Ostresh, J.M., Dooley, C.T., Houghten, R.A., Eichler, J.
  5. Mu opioid receptor ligands: Agonists and antagonists. U.S. Patent No. 5,919,897. Issued July 6, 1999.  Inventors:  Dooley, C.T., Houghten, R.A.
  6. μ Opioid receptor ligands: agonists and antagonists. U.S. Patent No. 5,641,861. Issued  June 24, 1997.  Inventors:  Dooley, C.T., Houghten, R.A.
  7. Kappa receptor selective opioid peptides. U.S. Patent No. 5,610,271. Issued March 11, 1997.  Inventors:  Dooley, C.T., Houghten, R.A.
  8. Opioid peptide inhibitors. U.S. Patent No. 5,367,053. Issued November 22, 1994.  Inventors:  Dooley, C.T., Houghten, R.A.
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