Torrey Pines Institute for Molecular Studies science image
Torrey Pines Institute for
Molecular Studies
3550 General Atomics Court, 2-129
San Diego, CA 92121-1122
USA
Scientists
David Loskutoff
Member and Chairman of Faculty
Mechanisms of Human Disease

858.909.5047 - phone
858.455.3804 - fax

Current Research

Using murine models and cell culture to explore the role of plasminogen activator inhibitor-1 (PAI-1) and the leptin/leptin receptor system in the various pathologies associated with obesity and type II diabetes (i.e., NIDDM)

Education

  • 1965 - B.A. Degree, Biological Sciences, University of California, Berkeley, CA
  • 1972 - Ph.D. Degree, Molecular Biology, University of Colorado, Boulder, CO

Academic Positions

  • 2006 – present - Member, Torrey Pines Institute for Molecular Studies, San Diego, CA
  • 200I-2005 - Professor, Department of Cell Biology, Division of Vascular Biology,
    The Scripps Research Institute, La Jolla, California
  • 1993-2001 - Chairman and Professor, Department of Vascular Biology,
    The Scripps Research Institute, La Jolla, California
  • 1990-1992 - Member, Committee for the Study of Vascular Biology,
    Scripps Clinic and Research Foundation, La Jolla, California
  • 1981-1990 - Associate Member, Committee for the Study of Vascular Biology,
    Scripps Clinic and Research Foundation, La Jolla, California
  • 1977 -1981 - Assistant Member, Department of Molecular Immunology,
    Scripps Clinic and Research Foundation, La Jolla, California
  • 1975-1977 - Research Associate, Department of Molecular Immunology,
    Scripps Clinic and Research Foundation, La Jolla, California
  • 1974-1975 - Research Associate,
    The Rockefeller University, New York, New York
  • 1992-1974 - Fellow, Damon Runyon Fund for Cancer Research,
    The Rockefeller University, New York, New York

Honors/Awards

  • Plenary Lecture, XIlth Congress of the International Society of Thrombosis and Hemostasis,
    Tokyo, Japan (1989)
  • Plenary Lecture, Xth International Congress on Fibrinolysis, Indianapolis, IN (1990)
  • ICF Organon Technika Prize for fundamental research in the area of fibrinolysis (1992)
  • State-of-the-Art Lecture, XIVth International Congress on Thrombosis and Haemostasis,
    New York, NY (1993)
  • Plenary Lecture, Keystone Symposia Joint Sessions on "Molecular Biology of the Endothelial Cell"; and on "Inflammation, Growth Regulatory Molecules & AtherosclerosIs", Keystone, CO (1994)
  • Elected Honorary Member for Life in the American Society for Clinical Investigation (1995)
  • Investigator Recognition Award for Contributions to Hemostasis (1995)
  • Member, AHA Council on Thrombosis, Executive Committee (1996)
  • International Advisory Committee for the XVlth Congress of the ISTH, Washington, D.C. (1999)
  • State-of-the-Art Lecture, XVllth Congress of the ISTH, Washington, D.C. (1999)
  • Pia-Glas Greenwalt Lectureship Award, XVllth Congress of the ISTH, Washington, D.C. (1999)
  • Fellow of the American Heart Association, The Council on Arteriosclerosis,
    Thrombosis, and Vascular Biology (2001)
  • Distinguished Career Award, XIXth Congress of the International Society on Thrombosis and Haemostasis, Birmingham, England (2003)
  • Plenary Lecture, Xth International Workshop on the Molecular and Cellular Biology of Plasminogen Activation, Washington, DC (2005)

Publications

  1. Konstantinides, S., Ware, J., Marchese, P., Loskutoff, D.J., and Ruggeri, Z.M. Distinct antithrombolic effects of platelet glycoprotein 1bα and VI deficiency in a mouse model of arterial thrombosis. J. Thromb. Haemost. 4: 2014-2021 (2006).
  2. Kamikuko, Y., Kroon, J., Curriden, S.A., Dyson, H. J., Loskutoff, D.J. The reduced and denatured form of the somatomedin B domain of vitronectin refolds into a stable, biologically active molecule. Biochem. 45: 3297-3306, 2006.
  3. Yepes, M., Loskutoff, D.J., Lawrence, D.A. Plasminogen Activator Inhibitor-1. In: Hemostasis and Thrombosis: Basic Principles and Clinical Practice. Fifth Edition. Eds. Drs. R.W. Colman, J. Hirsh, V.J. Marder, A.W. Clowes, J.N. George. Lippincott, Williams and Wilkens Publishers. Chapter 19, pages 365-380, 2006.
  4. Campagnolo, L., Leahy, A., Chitnis, S., Koschnick, S., Fitch, M.J., Fallon, J.T., Loskutoff, D.J., Taubman, M.B., Stuhlmann, H. EGFL7 is a chemoattractant for endothelial cells and is up-regulated in angiogenesis and arterial injury. Am. J. Pathol. 167: 275-284, 2005.
  5. Degryse, B., Resnati, M., Czekay, R.P., Loskutoff, D.J., Blasi, F. Domain 2 of the urokinase receptor contains an integrin-interacting epitope with intrinsic signaling activity: Generation of a new integrin inhibitor. J. Biol. Chem. 280: 24792 -24803, 2005.
  6. Dellas, C. and Loskutoff, D.J. Historical analysis of PAI-1 from its discovery to its potential role in cell motility and disease. Thromb. Haemost. 93:631-640, 2005.
  7. Giandomenico, G., Dellas, C., Czekay, R.P., Koschnick, S., Loskutoff, D.J. The leptin receptor system of human platelets. J. Thromb. Haemos. 3:1042-1049, 2005.
  8. Koschnick, S., Konstantinides, S., Schäfer, K., Crain, K., Loskutoff, D.J. Thrombotic phenotype of mice with a combined deficiency in plasminogen activator inhibitor-1 and vitronectin. J. Thromb. Haemos. 3:2290-2295, 2005.
  9. Pandey, M., Loskutoff, D.J., and Samad, F. Molecular mechanisms of tumor necrosis factor α-mediated plasminogen activator in ihibitor-1 expression in adipocytes. The FASEB Journal express article 10.1096/fj.04-3459fje, 2005.
  10. Pandey, M., Loskutoff, D.J., Samad, F. Molecular mechanisms of tumor necrosis factor-α-mediated plasminogen activator inhidotor-1 expression in adipocytes. The FASEB Journel express article 10.1096/fj.04-3459fje.Published online May 31 (2005).
  11. Pandey, M., Loskutoff, D.J., Samad, F. Molecular mechanisms of tumor necrosis factor-α-mediated plasminogen activator inhibitor-1 expression in adipocytes. FASEB Journal express article 10.1096/fj.04-3459fje, published online May 31, 2005.
  12. Yamamoto, K., Kojima, T., Adachi, T., Hayashi, M. Matsushiti, T., Takamatsu, J., Loskutoff, D.J., Saito, H. Obesity enhances the induction of plasminogen activator inhibitor-1 by restrain stress: A possible mechanism of stress-induced renal fibrin deposition in obese mice. J. Thromb. Haemost. 3:1495-1502, 2005.
  13. Czekay, R.P. and Loskutoff, D.J. Unexpected role of plasminogen activator inhibitor-1 in cell adhesion and detachment. Symposium Exp. BioI. Med. 229:1090-1096, 2004.
  14. Degryse, B., Neels, J.G., Czekay, R.P., Aertgeerts, K., Kamikubo, Loskutoff, D.J. The low density lipoprotein receptor-related protein is a motogenic receptor for plasminogen activator inhibitor-1. J. Biol. Chem. 279:22595-22604, 2004.
  15. Kamikubo, Y., De Guzman, R., Kroon, G., Curriden, S., Neels, J .G., Churchill, M.J., Dawson, P., Oldziej, S., Jagielska, A., Scheraga, H.A., Loskutoff, D.J., Dyson, H.J. Disulfide bonding arrangements in active forms of the somatomedin B domain of human vitronectin. Biochemistry 43:6519-6534, 2004.
  16. Konstantinides, S., Schäfer, K., Neels, J.G., Dellas, C., Loskutoff, D.J. Inhibition of endogenous leptin protects mice from arterial and venous thrombosis. Arterioscler Thromb. Vasc. BioI. 24:2196-2201, 2004.
  17. Neels, J.G., Thinnes, T., Loskutoff, D.J. Angiogenesis in an in vivo model of adipose tissue development. FASEB J. 10.1096/fj.03-1101fje, http://www.fasebj.org/cgi/reprint/03-1101Fjevl, 2004.
  18. Schäfer, K., Halle, M., Goeschen, C., Dellas, C., Pynn, Loskutoff, D.J., Konstantinides, S. Leptin promotes vascular remodeling and neointimal growth in mice. Arterioscler Thromb. Vasc. Biol. 24: 112-117, 2004.
  19. Takeshita, K., Hayashi, M., lino, S., Kondo, T., Inden, Y., Iwase, M., Kojima, T., Hirai, M., Ito, M., Loskutoff, D.J., Saito, H., Murohara, T., Yamamoto, K. Increased expression of plasminogen activator inhibitor-1 in cardiomyocytes contributes to cardiac fibrosis after myocardial infarction. Am. J. Pathol. 164:449-456, 2004.
  20. Czekay, R.P., Aertgeerts, K., Curriden, S.A., Loskutoff, D.J. Plasminogen activator inhibitor-1 detaches cells from extracellular matrices by inactivating integrins. J. Cell BioI. 160:781-791, 2003.
  21. Deng, G.G., Martin-McNulty, B., Sukovich, D.A., Freay, A., Halks-Miller, M., Thinnes, V.T., Loskutoff, D.J., Carmeliet, P., Dole, W.P., Wang, Y.X. Urokinase-type plasminogen activator plays a critical role in angiotensin ll-induced abdominal aortic aneurysm. Circulation Research 92:510-517, 2003.
  22. Gutierrez-Fernandez, A., Gingles, N.A., Zhang, L., Garcia Bannach, F., Jenkins, G.R., Loskutoff, D.J., Parmer, R.J., Miles, L.A. Regulation of plasminogen gene expression. Plasminogen: Structure, Activation, and Regulation. D.M. Waisman, editor. Kluwer Academic Plenum Publishers, New York, NY. 67-80, 2003.
  23. Sartipy, P. and Loskutoff, D.J. Monocyte chemoattractant protein 1 is overexpressed in murine obesity and may contribute to the development of insulin-resistance. Proc. Nat. Acad. Sci.-USA. 100:7265-7270, 2003.
  24. Sartipy, P. and Loskutoff, D.J. Expression profiling identifies genes that continue to respond to insulin in adipocytes made insulin-resistant by treatment with tumor necrosis factor-α. J. Biol. Chem. 278:52298-52306, 2003.
  25. Schafer, K., Muller, K., Hecke, A., Mounier, E., Goebel, J., Loskutoff, D.J., Konstantinides, S. Enhanced thrombosis in atherosclerosis-prone mice is associated with increased arterial expression of PAI-1. Arterioscler Thromb Vasc BioI. 23:2097 - 2103, 2003.
  26. Tarui, T., Andronicos, N., Czekay, R.P., Mazar, A.P., Bdeir, K., Parry, G.C., Kuo, A., Loskutoff, D.J., Cines, D.B., Takada, Y. Critical role of integrin á5â1 in urokinase (uPA)/urokinase receptor (uPAR, CD87) signaling. J. Biol. Chem. 278:29863-29872, 2003.
  27. Kamikubo, Y., Okumura, Y., Loskutoff, D.J. Identification of the disulfide bonds in the recombinant somatomedin B domain of human vitronectin. J. BioI. Chem. 277:27109- 27119, 2002.
  28. Konstantinides, S., Schafer, K., Loskutoff, D.J. Do PAI-1 and vitronectin promote or inhibit neointima formation: The exact role of the fibrinolytic system in vascular remodeling remains uncertain. Arterioscler Thromb Vasc BioI. 22:1943-1945, 2002.
  29. Loskutoff, D.J. Alterations in hemostatic gene expression in obesity. In: Vascular Protection: Molecular Mechanisms, Novel Therapeutic Principles and Clinical Application. Volume 9. Eds. Drs. G.M. Rubanyi, V.J. Dzau, J.P. Cook. Taylor & Francis, New York. Chapter 28, pages 347-357, 2002.
  30. Okumura, Y., Kamikubo, Y., Curriden, S., Wang, J., Kiwada, T., Futaki, S., Kitagawa, K., Loskutoff, D.J. Kinetic analysis of the interaction between vitronectin and the urokinase receptor. J. Biol. Chem. 277:9395-9404, 2002.
  31. Schafer, K., Konstantinides, S., Riedel, C., Thinnes, T., Müller, K., Dellas, C., Hasenfuss, G., Loskutoff, D.J. Different mechanisms of increased luminal stenosis after arterial injury in mice deficient for urokinase- or tissue-type plasminogen activator. Circulation 106:1847-1852, 2002.
  32. Takeshita, K., Yamamoto, K., Ito, M., Kondo, T., Matsushita, T., Hirai, M., Kojima, T., Nishimura, M., Nabeshima, Y., Loskutoff, D.J., Saito, H., Murohara, T. Increased expression of plasminogen activator inhibitor-1 with fibrin deposition in a murine model of aging, "klotho" mouse. Semin. Thromb. Hemost. 28:545-554, 2002.
  33. Yamamoto, K., Shimokawa, T., Yi, H., Isobe, K.I., Kojima, T., Loskutoff, D.J., Saito, H. Aging accelerates endotoxin-induced thrombosis: increased responses of plasminogen activator inhibitor-1 and LPS signaling with aging. Am. J. Pathol. 161:1805-1814, 2002.
  34. Yamamoto, K., Shimokawa, T., Yi, H., Isobe, K.-i., Kojima, T., Loskutoff, D.J., Saito, H. Aging and obesity augment the stress induced expression of tissue factor gene in the mouse. Blood 100:4011-4018, 2002.
  35. Yamamoto, K., Takeshita, K., Shimokawa, T., Yi, H., Isobe, K., Loskutoff, D.J., Saito, H. Plasminogen activator inhibitor-l is a major stress-regulated gene: Implications for stress- induced thrombosis in aged individuals. Proc. Natl. Acad. Sci. USA, 99:890-895, 2002.
  36. Zhang, L., Seiffert, D., Fowler, B.J., Jenkins, G.R., Thinnes, T.C., Loskutoff, D.J., Parmer, R.J., Miles, L.A. Plasminogen has a broad extrahepatic distribution. Thromb. Haemost. 87:493-501, 2002.
  37. Bajou, K., Masson, V., Gerard, R.D., Schmitt, P.M., Albert, V., Praus, M., Lund, L.R., Frandsen, T.L., Brunner, N., Dano, K., Fusenig, N.E., Weidle, V., Carmeliet, G., Loskutoff, D.J., Collen D., Carmeliet, P., Foidart, J.M., Noel, A. The plasminogen activator inhibitor PAI-1 controls in vivo tumor vascularization by interaction with proteases, not vitronectin: implications for antiangiogenic strategies. J. Cell Biology 152: 777-784, 2001.
  38. Deng, G., Curriden, S.A., Hu, G., Czekay, R.-P., Loskutoff, D.J. Plasminogen activator inhibitor-1 regulates cell adhesion by binding to the somatomedin B domain of vitronectin. J.Cell.Physiol. 189:23-33, 2001.
  39. Hollestelle, M.J., Thinnes, T., Crain, K., Stiko, A., Kruijt, J.K., van Berkel, T.J.C., Loskutoff, D.J., van Mourik, J.A. Tissue distribution of Factor VIII gene expression in vivo; A closer look. Thromb. Haemost. 86:855-862, 2001.
  40. Ihara, H., Vrano, T., Takada, A., Loskutoff, D.J. Induction of plasminogen activator inhibitor-1 (PAI-1) gene expression in adipocytes by thiazolidinediones. FASEB J. (March 5, 2001) 10.1096/fj.00-0570fje, 2001.
  41. Konstantinides, S., Schafer, K., Koschnick, K., Loskutoff, D.J. Leptin-dependent platelet aggregation and arterial thrombosis suggests a mechanism for atherothrombotic disease in obesity. J. Clinical Invest. 108:1533-1540, 2001.
  42. Konstantinides, S., Schafer, K., Loskutoff, D.J. The Prothrombotic Effects of Leptin: Possible Implications for the Risk of Cardiovascular Disease in Obesity. Ann. N.Y. Acad. Sci. 947:134-142, 2001.
  43. Konstantinides, S., Schafer, K., Thinnes, T., Loskutoff, D.J. PAI-1 and its cofactor vitronectin stabilize arterial thrombi following vascular injury in mice. Circulation 103: 576-583, 2001.
  44. Royle, G., Deng, G., Seiffet, D., Loskutoff, D.J. A method for defining binding sites involved in protein-protein interactions: Analysis of the binding of plasminogen activator inhibitor-1 to the somatomedin domain of vitronectin. Anal. Biochem. 296:245-253, 2001.
  45. Samad, F., Pandey, M and Loskutoff, D.J. Regulation of tissue factor gene expression in obesity. Blood 98(12):3353-3358, 2001.
  46. Samad, F., Pandey, M., Loskutoff, D.J. Regulation of tissue factor gene expression in obesity. Blood 98:3353-3358, 2001.
  47. Schafer, K., Fujisawa, K. Loskutoff, D.J. Disruption of the plasminogen activator inhibitor-1 gene reduces the adiposity and improves the metabolic profile of genetically obese and diabetic ob/ob mice. FASEB J. (June 27, 2001) 10.1096/fj.00-0750fje, 2001.
  48. Loskutoff, D.J. and Quigley, J.P. PAI-1, fibrosis and the elusive provisional fibrin matrix. J. Clin Invest. 106:1441-1443, 2000.
  49. Loskutoff, D.J., Fujisawa, K., Samad, F. The fat mouse: A powerful genetic model to study hemostatic gene expression in obesity/NIDDM. Atherosclerosis V; The Fifth Saratoga International Conference. Fujio Numano, Michael A. Gimbrone, Jr. (eds). Annals N.Y. Acad. Sci. Vol. 902: 272-282, 2000.
  50. Loskutoff, D.J., Ihara, I., Yamamoto, K. Samad, F. The fat mouse: A powerful genetic model to study hemostatic gene expression in obesity. New Frontier in Vascular Biology; Thrombosis and Hemostasis, Suzuki, K., Ikeda, Y. and Maruyama, I. (ed.) Eibun Press, Ltd., Osaka, Japan, 2000.
  51. Samad, F., Pandey M., Bell, P.A., Loskutoff, D.J. Insulin continues to induce plasminogen activator inhibitor 1 gene expression in insulin-resistant mice and adipocytes. Mol. Med. 6: 680-692, 2000.
  52. Samad, F., Pandey,M. and Loskutoff, D.J. Insulin continues to induce plasminogen activator inhibitor 1 gene expression in insulin-resistant mice and adipocytes. Molecular Medicine 6(8):680-692, 2000.
  53. Stiko, A., Hervio, L., Loskutoff, D.J. PAI-1 and other fibrinolytic inhibitors. Hemostasis and Thrombosis: Basic Principles and Clinical Practice. Fourth Edition. Eds. Drs. RW Colman, J Hirsh, VJ Marder, A W Clowes, JN George. Lippincott-Raven Publishers. Chapter 18, pages 355-365, 2000.
  54. Yamamoto, K. and Loskutoff, D. J. Expression of transforming growth factor-β and tumor necrosis factor-α in the plasma and tissues of mice with lupus nephritis. Laboratory Investigation 80: 1561-1570, 2000.
  55. Loskutoff, D.J., Curriden, S.A., Hu, G., Deng, G. Regulation of cell adhesion by PAl-1. APMIS. 107:54-61, 1999.
  56. Samad, F. and Loskutoff, D.J. Hemostatic gene expression and vascular disease in obesity: Insights from studies of genetically obese mice. Thromb. Haemost. 82:742-747, 1999.
  57. Samad, F., Hotamisligil, G.S., Uysal, K.T., Pandey, M., Weisbrock, S.M. and Loskutoff, D.J. Tumor Necrosis Factor-a: A key component in the obesity-linked elevation of plasminogen activator inhibitor-1. Proc. Natl. Acad. Sci. USA 96:6902-6907, 1999.
  58. Samad, F., Uysal, K.T., Wiesbrock, S.M., Pandey, M., Hotamisligil, G.S., Loskutoff, D.J. Tumor necrosis factor-α is a key component in the obesity-linked elevation of plasminogen activator inhibitor-1. Proc. Natl. Acad. Sci. USA. 96:6902-6907, 1999.
  59. Yamamoto, K., Shimokawa, T., Kojima, T., Loskutoff, D.J., Saito, H. Regulation of murine protein C gene expression in vivo: effects of tumor necrosis factor-α, interleukin-1, and transforming growth factor-β. Thromb. Haemost. 82:1297-1301, 1999.
  60. Estelles, A., Gilabert, J., Grancha, S., Yamamoto, K., Thinnes, T., Espana, F., Aznar, J., Loskutoff, D.J. Abnormal expression of type 1 plasminogen activator inhibitor and tissue factor in severe preeclampsia. Thromb. Haemost. 79:500-508, 1998.
  61. Loskutoff, D.J. and Samad, F. The adipocyte and hemostatic balance in obesity: Studies of plasminogen activator inhibitor 1. Arterioscler Thromb Vasc BioI. 18: 1-6, 1998.
  62. Pinsky, D.J., Liao, H., Lawson, C.A., Yan, S.-F., Chen, J., Carmeliet, P., Loskutoff, D.J., Stern, D.M. Coordinated induction of plasminogen activator inhibitor-1 (PAI-1) and inhibition of plasminogen activator gene expression by hypoxia promotes pulmonary vascular fibrin deposition. J. Clin. Invest. 102:919-928, 1998.
  63. Samad, F., Pandey, M. and Loskutoff, D.J. Tissue factor gene expression in the adipose tissues of obese mice. Proc. Natl. Acad. Sci. USA. 95:7591-7599, 1998.
  64. Samad, F., Pandey, M., Loskutoff, D.J. Tissue factor gene expression in the adipose tissues of obese mice. Proc. Natl. Acad. Sci. USA. 95:7591-7596, 1998.
  65. Schneiderman, J., Bordin, G.M., Adar, R., Smolinsky, A., Seiffert, D., Engelberg, I., Dilley, R.B., Thinnes, T., Loskutoff, D.J. Patterns of expression of fibrinolytic genes and matrix metalloproteinase-9 in dissecting aortic aneurysms. Am. J. Pathol. 152:703-710,1998.
  66. Yamamoto, K. and Loskutoff, D.J. Extrahepatic expression and regulation of protein C in the mouse. Am. J. Pathol. 153:547-555, 1998.
  67. Yamamoto, K., de Waard, V., Fearns, C., Loskutoff, D.J. Tissue distribution and regulation of murine von Willebrand factor gene expression in vivo. Blood 92:2791-2801, 1998.
  68. Yamamoto, K., Loskutoff, D.J., Saito, H. Renal expression of fibrinolytic genes and tissue factor in a murine model of renal disease as a function of age. Sem. Thromb. Hemostas. 24:261-268, 1998.
  69. Fearns, C. and Loskutoff, D.J. Induction of plasminogen activator inhibitor 1 gene expression in murine liver by lipopolysaccharide: Cellular localization and role of endogenous tumor necrosis factor-α. Am. J. Pathol. 150:579-590, 1997.
  70. Fearns, C. and Loskutoff, D.J. Role of tumor necrosis factor alpha in induction of murine CD14 gene expression by lipopolysaccharide. Infect. Immun. 65:4822-4831, 1997.
  71. Lawrence, D.A. and Loskutoff, D.J. Plasminogen activator inhibitor type-1. In: Human Protein Data. Haeberli, A. (ed). WILEY -VCH Verlag GmbH, Weinheim, 1997.
  72. Loskutoff, D.J. PAI-1 inhibits neointimal formation after arterial injury in mice - A new target for controlling restenosis? Circulation 96:2772-2774, 1997.
  73. Loskutoff, D.J. and Samad, F. Mechanisms of elevated plasminogen activator inhibitor 1 in obesity. Takada, A., Collen, D., Gaffney, P.J. eds. Recent Progress in Blood Coagulation and Fibrinolysis; International Congress Series 1129, EXCERPTA MEDICA ELSEVIER, pp 221-227, 1997.
  74. Samad, F. and Loskutoff, D.J. The fat mouse: A powerful genetic model to study elevated plasminogen activator inhibitor 1 in obesity/NIDDM. Thromb. Haemost. 78:652-655, 1997.
  75. Samad, F., Schneiderman, J., Loskutoff, D.J. Expression of fibrinolytic genes in tissues from human atherosclerotic aneurysms and from obese mice. Ann. NY Acad. Sci. 811,350-360, 1997.
  76. Samad, F., Yamamoto, K., Pandey, M. and Loskutoff, D.J. Elevated expression of transforming growth factor-β in adipose tissue from obese mice. Mol. Med. 3:37-48, 1997.
  77. Samad, F., Yamamoto, K., Pandey, M., Loskutoff, D.J. Elevated expression of transforming growth factor-ƒÒ in adipose tissue from obese mice. Mol. Med. 3:37-48, 1997.
  78. van Aken, B.E., Seiffert, D., Thinnes, T., Loskutoff, D.J. Localization of vitronectin in the normal and atherosclerotic human vessel wall. Histochem. Cell BioI. 107:313-320, 1997.
  79. Yamamoto, K. and Loskutoff, D.J. The kidneys of mice with autoimmune disease acquire a hypofibrinolytic/procoagulant state that correlates with the development of glomerulonephritis and tissue microthrombosis. Am. J. Pathol. 151:725-734, 1997.
  80. Deng, G., Curriden, S.A., Wang, S., Rosenberg, S., Loskutoff, D.J. Is plasminogen activator inhibitor-1 the molecular switch that governs urokinase receptor mediated cell adhesion and release? J. Cell BioI. 134:1563-1571, 1996.
  81. Deng, G., Royle, G., Wang, S., Crain, K., Loskutoff, D.J. Structural and functional analysis of the PAI-1 binding motif in the somatomedin B domain of vitronectin. J. BioI. Chem. 271:12716-12723, 1996.
  82. Estelles, A., Grancha, S., Gilabert, J., Thinnes, T., Chirivella, M., Espana, F., Aznar, J., Loskutoff, D.J. Abnormal expression of plasminogen activator inhibitors in patients with gestational trophoblastic disease. Am. J. Pathol. 149:1229-1239, 1996.
  83. Fearns, C., Samad, F., Loskutoff, D.J. Synthesis and localization of PAI-1 in the vessel wall. Vascular Control of Hemostasis. In: Advances in Vascular Biology, Vadas, M., Harlan, J. (eds). Gordon and Breach Publishers, Camberwell, Victoria, Australia p. 207- 226, 1996.
  84. Luther, T., Flössel, C., Mackman, N., Bierhaus, A., Kasper, M., Albrecth, S., Sage, E.H., Iruela-Arispe, L., Grossmann, H., Strahlein, A., Zhang, Y., Nawroth, P.P., Carmeliet, P., Loskutoff, D.J., Müller, M. Tissue factor expression during human and mouse development. Am. J. Pathol. 149:101-113, 1996.
  85. Samad, F. and Loskutoff, D.J. Tissue distribution and regulation of plasminogen activator inhibitor 1 in obese mice. Mol. Med. 2:568-582, 1996.
  86. Samad, F. and Loskutoff, D.J. Tissue distribution and regulation of plasminogen activator inhibitor-l in obese mice. Mol. Med. 2:568-582, 1996.
  87. Samad, F., Yamamoto, K. and Loskutoff, D.J. Distribution and regulation of plasminogen activator inhibitor-1 in murine adipose tissue in vivo: Induction by tumor necrosis factor-a and lipopolysaccharide. J. Clin. Invest. 97:37-46, 1996.
  88. Samad, F., Yamamoto, K., Loskutoff, D.J. Distribution and regulation of plasminogen activator inhibitor-l in murine adipose tissue in vivo: Induction by tumor necrosis factor-Ą and lipopolysaccharide. J. Clin. lnvest. 97:37-46, 1996.
  89. Schneiderman, J., Adar, R., Engelberg, I., Bordin, G.M., Seiffert, D., Loskutoff, D.J., Dilley, R.B. Medical control of abdominal aortic aneurysm expansion rate. J. Vasc. Surg. 24:297, 1996.
  90. Seiffert, D. and Loskutoff, D.J. Type 1 plasminogen activator inhibitor induces multimerization of plasma vitronectin: A suggested mechanism for the generation of the tissue-form of vitronectin in vivo. J. BioI. Chem. 271:29644-29651, 1996.
  91. Seiffert, D., Bordin, G.M., Loskutoff, D.J. Evidence that extrahepatic cells express vitronectin mRNA at rates approaching those of hepatocytes. Histochem. Cell BioI. 105:195-201, 1996.
  92. Seiffert, D., Curriden, S.A., Jenne, D., Binder, B.R., Loskutoff, D.J. Differential regulation of vitronectin in mice and humans in vitro. J. BioI. Chem. 271:5474-5480, 1996.
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  201. Sawdey, M., Ny, T., Loskutoff, D.J. Messenger RNA for plasminogen activator inhibitor. Thromb. Res. 41:151-160, 1986.
  202. Schleef, R.R., Wagner, N.V., Sinha, M., Loskutoff, D.J. A monoclonal antibody that does not recognize tissue-type plasminogen activator bound to its naturally occurring inhibitor. Thromb. Haemost. 56:328-332, 1986.
  203. Erickson, L.A., Ginsberg, M.H., Loskutoff, D.J. An inhibitor of tissue-type plasminogen activator in human platelets, serum, and plasma. Prog. in Fibrinolysis 7:137-140, 1985.
  204. Erickson, L.A., Hekman, C.M., Loskutoff, D.J. The primary plasminogen-activator inhibitors in endothelial cells, platelets, serum, and plasma are immunologically related. Proc. Natl. Acad. Sci. USA. 82:8710-8714,1985.
  205. Erickson, L.A., Schleef, R.R., Ny, T., Loskutoff, D.J. The fibrinolytic system of the Vascular Wall. Clinics in Hematology 14:513-530, 1985.
  206. Hekman, C.M. and Loskutoff, D.J. Endothelial cells produce a latent inhibitor of plasminogen activators that can be activated by denaturants. J. BioI. Chem. 260: 11581-11587, 1985.
  207. Loskutoff, D.J. The fibrinolytic system of cultured endothelial cells: Deciphering the balance between plasminogen activation and inhibition. Prog. in Fibrinolysis 7:15-22, 1985.
  208. Ny, T., Bjersing, L., Hsueh, A.J.W., Loskutoff, D.J. Cultured granulosa cells produce two plasminogen activators and an antiactivator, each regulated differently by gonadotropins. Endocrinology 116:1666-1668, 1985.
  209. Sakata, Y., Curriden, S., Lawrence, D., Griffin, J.H., Loskutoff, D.J. Activated protein C stimulates the fibrinolytic activity of cultured endothelial cells and decreases antiactivator activity. Proc. Natl. Acad. Sci. USA. 82:1121-1125, 1985.
  210. Schleef, R.R., Sinha, M., Loskutoff, D.J. Characterization of two monoclonal antibodies against human tissue-type plasminogen activator. Thromb. Haemost. 53:170-175, 1985.
  211. Schleef, R.R., Sinha, M., Loskutoff, D.J. Immunoradiometric assay to measure the binding of a specific inhibitor to tissue-type plasminogen activator. J. Lab. and Clin. Med. 106:408- 415, 1985.
  212. Erickson, L.A., Ginsberg, M.H., Loskutoff, D.J. Detection and partial characterization of an inhibitor of plasminogen activator in human platelets. J. Clin. Invest. 74: 1465-1472, 1984.
  213. Erickson, L.A., Lawrence, D.A., Loskutoff, D.J. Reverse fibrin autography: A method to detect and partially characterize protease inhibitors after sodium dodecyl sulfate- polyacrylamide gel electrophoresis. Anal. Biochem. 137:454-463, 1984.
  214. Loskutoff, D.J. and Levin, E. Properties of plasminogen activators produced by endothelial cells. The Biology of Endothelial Cells. Jaffe, E.A. (ed). Martinus Nijhoff Publishers, The Netherlands, p. 200-208, 1984.
  215. Loskutoff, D.J. and Wiggins, R.C. Activation of Hageman factor by cultured rabbit endothelial cells. The Biology of Endothelial Cells. Jaffe, E.A. (ed). Martinus Nijhoff Publishers, The Netherlands, p. 224-227, 1984.
  216. Mussoni, L., Lawrence, D.A., Loskutoff, D.J. A direct, plasmin-independent assay for plasminogen activator. Thromb. Res. 34:241-254, 1984.
  217. van Mourik, J.A., Lawrence, D.A., Loskutoff, D.J. Purification of an inhibitor of plasminogen activator (antiactivator) synthesized by endothelial cells. J. BioI. Chem. 259:14914-14921, 1984.
  218. Levin, E.G., Fair, D.S., Loskutoff, D.J. Human hepatoma cell line plasminogen activator. J. Lab. & Clin. Med. 102:500-508, 1983.
  219. Loskutoff, D.J. and Gerna, M. An inhibitor in rabbit endothelial cells that recognizes urokinase-like but not tissue type plasminogen activators. Prog. Fibrinolysis 6:394-396, 1983.
  220. Loskutoff, D.J. and Mussoni, L. Interactions between fibrin and the plasminogen activators produced by cultured endothelial cells. Blood 62:62-68, 1983.
  221. Loskutoff, D.J., Levin, E.G., Mussoni, L. Fibrin-binding properties of plasminogen activators produced by endothelial cells. Prog. Fibrinolysis 6:15-19, 1983.
  222. Loskutoff, D.J., van Mourik, J.A., Erickson, L.A., Lawrence, D. Detection of an unusually stable fibrinolytic inhibitor produced by bovine endothelial cells. Prod. Natl. Acad. Sci. USA 80:2956-2960,1983.
  223. Bykowska, K., Levin, E.G., Rijken, D.C., Loskutoff, D.J., Collen, D. Characterization of a plasminogen activator secreted by cultured bovine aortic endothelial cells. Biochem. Biophys. Acta. 703:113-115, 1982.
  224. Levin, E.G. and Loskutoff, D.J. Regulation of plasminogen activator production by cultured endothelial cells. Annals N.Y. Acad. Sci. 401:184-194, 1982.
  225. Levin, E.G. and Loskutoff, D.J. Regulation of plasminogen activator production by cultured endothelial cells. Annals N.Y. Acad. Sci. 401:184-194, 1982.
  226. Levin, E.G. and Loskutoff, D.J. Cultured bovine endothelial cells produce both urokinase and tissue-type plasminogen activators. J. Cell. BioI. 94:631-636, 1982.
  227. Loskutoff, D.J., Levin, E., Mussoni, L. Fibrinolytic components of cultured endothelial cells. Pathobiology of the Endothelial Cell. Nossel, H.L., Vogel, H. (eds). Academic Press, p. 167-182, 1982.
  228. Loskutoff, D.J., Levin, E.G., Mussoni, L. Fibrin-binding properties of plasminogen activators produced by endothelial cells. Progress in Fibrinolysis Davidson, J.F., et al (eds). p. 15-19, 1982.
  229. Loskutoff, D.J. Review of clotting and the differences between plasma and serum that might be significant to cell culture. The Growth Requirements of Vertebrate Cells In Vitro. Waymouth, C., Ham, R., Chapple, P.J. (eds). Cambridge University Press, p. 358- 374,1981.
  230. Loskutoff, D.J. and Edgington, T.S. An inhibitor of plasminogen activator in rabbit endothelial cells. J. BioI. Chem. 256:4142-4145,1981.
  231. Balk, S.D., Hoon, B.S., Loskutoff, D.J., Mitchell, R.S., Le Stourgeon, D.N., Weselake, R. Rapid, reversible rounding and aggregation of Rous Sarcoma Virus-infected chicken fibroblasts induced by a plasma macromolecule. Can. Res. 40:1753-1760, 1980.
  232. Levin, E.G. and Loskutoff, D.J. Serum-mediated suppression of cell-associated plasminogen activator activity in cultured endothelial cells. Cell 22:701-707, 1980.
  233. Loskutoff, D.J. and Levin, E.G. Fluctuations in the fibrinolytic activity of cultured endothelial cells caused by routine culture manipulations. Developments in Biochem. 8:589-595, 1980.
  234. Wiggins, R., Loskutoff, D.J., Cochrane, C.G., Griffin, J.H., Edgington, T.S. Activation of rabbit Hageman factor by homogenates of cultured rabbit endothelial cells. J. Clin. Invest. 65:197-206, 1980.
  235. Levin, E.G. and Loskutoff, D.J. Comparative studies of the fibrinolytic activity of cultured vascular cells. Thromb. Res. 15:869-878, 1979.
  236. Loskutoff, D.J. Effect of thrombin on the fibrinolytic activity of cultured bovine endothelial cells. J. Clin. Invest. 64:329-332, 1979.
  237. Shakalai, M., Loskutoff, D.J., Tavassoli, M. Membrane characteristics of cultured endothelial cells: Identification of gap function. Israel J. Med. Sci. 14:306-313, 1979.
  238. Andrews, B.S., Theofilopoulos, A.N., Peters, C.J., Loskutoff, D.J., Brandt, W.E., Dixon, F.J. Replication of dengue and junin viruses in cultured rabbit and human endothelial cells. Infect. and Immunity 20:776-781, 1978.
  239. Loskutoff, D.J. Effects of acidified fetal bovine serum on the fibrinolytic activity and growth of cells in culture. J. Cell. Physiol. 96:361-369, 1978.
  240. Loskutoff, D.J. Preparation of tissue culture serum lacking α-macgroblobulin. FEBS Letters 94:73-76, 1978.
  241. Loskutoff, D.J. and Paul, D. Intracellular plasminogen activator activity in growing and quiescent cells. J. Cell. Physiol. 97:9-16, 1978.
  242. Loskutoff, D.J. and Edgington, T.S. Synthesis of a fibrinolytic activator and inhibitor by endothelial cells. Proc. Natl. Acad. Sci. USA. 74:3903-3907, 1977.
  243. Rifkin, D.B., Gilula, N.B., Quigley, J.P., Loskutoff, D.J., Ossowski, L., Unkeless, J.E., Dano, K., Piperno, A. Proteases associated with malignant transformation. In: Mechanisms of Virus Diseases. Robinson, W.S., Fox, C.F. (eds). W.A. Benjamin Co., p.315-325, 1974.
  244. Loskutoff, D.J. and Pene, J.J. Gene expression during the development of Bacillus subtilis bacteriophage ø29. ll. Resolution of viral-specific ribonucleic acid molecules. J. Virol. 11:87-97, 1973.
  245. Loskutoff, D.J., Pene, J.J., Andrews, D.P. Gene expression during the development of Bacillus subtilis bacteriophage ø29. I. Analysis of viral-specific transcription by deoxyribonucleic acid, ribonucleic acid competition hybridization. J. Virol. 11:78-86, 1973.
  246. Dallman, P.R. and Loskutoff, D.J. Cytochrome oxidase repair during treatment of copper deficiency: Relation to mitochondrial turnover. J. Clin. Invest. 46:1819-1827, 1967.
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