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CALIFORNIA SCIENTISTS James Binley Todd Braciak Yang D. Dai Joanna Davies Brigitte Dudouet Richard A. Houghten Tony Hugli Valeria Judkowski J. Patrick Kampf Sophia Khaldoyanidi Alan Kleinfeld Prasad Koka Vipin Kumar Eugene G. Levin David Loskutoff Adel Nefzi Ron Ogata Clemencia Pinilla Claudia Raja Gabaglia Roy Riblet Fahu Samad Sally Sarawar Eli Sercarz Ingrid Schraufstatter Darcy Wilson FLORIDA SCIENTISTS Christopher Armishaw Colette Dooley Marc Giulianotti Richard A. Houghten Jose Luis Medina-Franco Karina Martinez Mayorga

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Dr. Kampf completed his undergraduate studies in 1995 at Carnegie Mellon University and received his Ph.D. in 2000 from Stanford University, both in chemical engineering. In 1999, he was a Research Intern at ALZA Corporation, in Mountain View, CA and completed his postdoctoral studies in membrane biology at Torrey Pines Institute for Molecular Studies. In 2002, he was named Senior Scientist in Membrane Biology at TPIMS and the Director of Research in Biophysics at FFA Sciences.

Research Focus

In collaboration with Dr. Alan Kleinfeld and FFA Sciences, Dr. Kampf studies fatty acid membrane transport and disease-mediated alterations in serum fatty acid profiles using fluorometry and kinetic modeling.

Honors

NIH Graduate Trainee in Biotechnology, 1997 - 1999.

Publications

1. Kampf JP, Parmley D, Kleinfeld AM. Free fatty acid transport across adipocytes is mediated by an unknown membrane protein pump. Am J Physiol Endocrinol Metab. 2007 Nov;293(5):E1207-14.
2. Kampf JP, Kleinfeld AM. Is membrane transport of FFA mediated by lipid, protein, or both? An unknown protein mediates free fatty acid transport across the adipocyte plasma membrane. Physiology.(Bethesda.) 2007 Feb;22:7-14.
3. Huber AH, Kampf JP, Kwan T, Zhu B, Kleinfeld AM. Fatty acid-specific fluorescent probes and their use in resolving mixtures of unbound free fatty acids in equilibrium with albumin. Biochemistry 2006 Dec 5;45(48):14263-74.
4. Lechene, C., Hillion, F., McMahon, G., Benson, D., Kleinfeld, A.M., Kampf, J.P., Distel, D., Luyten, Y., Bonventre, J., Hentschel, D., Park, K.M., Ito, S., Schwartz, M., Benichou, G., Slodzian, G. High resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry. J. Biol., 5, 20 (2006).
5. Kampf,J.P., Cupp,D. & Kleinfeld,A.M. Different mechanisms of free fatty acid flip-flop and dissociation revealed by temperature and molecular species dependence of transport across lipid vesicles. J. Biol. Chem., 281, 21566-21574 (2006).
6. Kleinfeld,A.M., Kampf,J.P. & Lechene,C. Transport of 13C-oleate in adipocytes measured using multi imaging mass spectrometry. J. Am. Soc. Mass Spectrom. 15, 1572-1580 (2004).
7. J. P. Kampf, A. M. Kleinfeld, "Fatty Acid Transport in Adipocytes Monitored by Imaging Intracellular FFA Levels," J. Biol. Chem., 279, 35775 (2004).
8. D. Cupp, J. P. Kampf, A. M. Kleinfeld, "Fatty Acid:Albumin Complexes and the Determination of Long Chain Free Fatty Acid Transport Across Membranes," Biochemistry, 43, 4473 (2004).
9. J. P. Kampf, C. W. Frank, "Chemical Sensors, Dendritic Polymer-based," in Encyclopedia of Materials: Science and Technology, K.H.J. Buschow, et al, ed., Elsevier: Amsterdam, 2001, pp. 1169-1173.
10. J. P. Kampf, C. W. Frank, E. E. Malmström, C. J. Hawker, "Adaptation of Bulk Constitutive Equations to Insoluble Monolayer Collapse at the Air-Water Interface," Science, 283, 1730 (1999).
11. J. P. Kampf, C. W. Frank, E. E. Malmström, C. J. Hawker, "Stability and Molecular Conformation of Poly(benzyl ether) Monodendrons with Oligo(ethylene glycol) Tails at the Air-Water Interface," Langmuir, 15, 227 (1999).
12. J. P. Kampf, T. L. Einloth, C. W. Frank, Y. Li, J. M. Oh, J. A. Moore, "Pentaerythritol-Based Polyether Dendrimers at the Air-Water Interface," in Organic Thin Films: Structure and Application, C.W. Frank, ed., American Chemical Society: Washington, D.C., 1998, pp. 31-42.
13. C. J. Hawker, E. E. Malmström, C. W. Frank, J. P. Kampf, "Exact Linear Analogs of Dendritic Polyether Macromolecules: Design, Synthesis, and Unique Properties," J. Am. Chem. Soc., 119, 9903 (1997).
14. J. P. Kampf, P. J. Sides, G. W. D. Spratt, "Reproducible Direct Exposure Testing of Metal Evaporated Media," J. Electrochem. Soc., 142, 3550 (1995).
15. P. J. Sides, G. Spratt, J. P. Kampf, "An Investigation of the Archivability of Metal Particle Tape," IEEE Trans. Magn., 30, 4059 (1994).