Roy Riblet
Member
Genetics
858.455.3762 - phone
858.455.3739 - fax
YAC Contig
The mouse immunoglobulin heavy chain (Igh) complex
locus is composed of more than 100 gene segments encoding
the variable, diversity, joining and constant portions
of the antibody heavy chain protein. To advance the
characterization of this locus and to identify all the
Vh genes, we have isolated the entire region from C57BL/6
and C57BL/10 as a Yeast Artificial Chromosome (YAC)
contig.
The mouse Igh locus extends approximately three megabases
and contains at least 134 Vh genes classified in 15
partially interspersed families. Two non-Igh pseudogenes
(Odc-rs8 and Rpl32-rs14) were localized in the distal
part of the locus. This physical YAC map will provide
important structure and guidance for the sequencing
of this large, complex and highly repetitive locus.
(Click on image for a more
detailed view)
A Table (PDF format) listing the sources, sizes, and
end sequences with GenBank accessions is HERE
The Southern blots of the YAC panels
probed with various Igh probes are below
Southern
blots of Igh locus YACs
(click
on link to open image in a new window)
centromere
O
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| Cmu (IgM) film
3201
|
| Jh [pJ11] film
3204
|
| Dh [DFL16.1] film
3139
|
| Vh7183 [81X] film
2120
|
| VhQ52 film
2112
|
| VhS107 film
2253
|
| VhSM7 film
2323
|
| VhX24 film
2190 film2195
|
| Vh11 film
2204
|
| Vh36-60 film
2076
|
| VhVGam3-8 film
2169
|
| Vh3609N film
3137
|
| Vh12 film
2177
|
| VhJ606 film
2315
|
| Vh10 film
2303
|
| Vh15 film
|
| Vh3609P film
2308
|
| VhJ558 film
3318
|
| Ocd-rs8 film
2613
|
| Rpl32-rs14 film
2648
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*
telomere |
This is a guide to the Southern blots of the
set of Yeast Artificial Chromosomes (YACs) that
contain the mouse Immunoglobulin Heavy Chain (Igh)
locus, as described in Chevillard et al. "A
3 megabase Yeast Artificial Chromosome contig
spanning the C57BL mouse Igh locus."
To the left is a schematic map of the Igh locus.
The Southern blots showing the content of each
gene or family of genes for constant region (C),
Joining region (Jh), Diversity region (Dh), and
Variable region (Vh) segments in the YACs are
identified by "film number". In each
blot figure the (EcoRI digested) YACs are listed
across the top, and marker sizes are listed on
the side. Clicking on the film number will open
the blot image.
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